Magnetic-activated cell sorting before density gradient centrifugation improves recovery of high-quality spermatozoa
نویسندگان
چکیده
منابع مشابه
Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution
Background: One of the factors limiting successful processing of alpaca (Vicugna pacos) semen is the viscosity of seminal plasma. The viscous nature of the collected ejaculate has hindered sperm cryopreservation as well as artificial insemination (AI) under field conditions. Aims: The objective of this investigation was to evaluate recovery, motility, ...
متن کاملEfficient isolation of sperm with high DNA integrity and stable chromatin packaging by a combination of density-gradient centrifugation and magnetic-activated cell sorting
OBJECTIVE This study was carried out to investigate the correlations of the sperm DNA fragmentation index (DFI) with semen parameters and apoptosis, and to investigate the effects of density-gradient centrifugation (DGC) and magnetic-activated cell sorting (MACS) on reducing the proportion of sperm with DNA fragmentation and protamine deficiency. METHODS Semen analysis and a sperm DNA fragmen...
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Background Sperm preparation before assisted reproductive techniques is very important for obtaining successful fertilization and pregnancy. During ICSI technique, sperms are selected based on viability and morphology. But this selection did not guarantee integrity of sperm DNA. Therefore, novel sperm selection procedures are introduced for selection of sperm with low DNA damage. One of these p...
متن کاملProcessing of semen by density gradient centrifugation selects spermatozoa with longer telomeres for assisted reproduction techniques.
The ends of eukaryotic chromosomes contain specialized chromatin structures called telomeres, the length of which plays a key role in early human embryonic development. Although the effect of sperm preparation techniques on major sperm characteristics, such as concentration, motility and morphology have been previously documented, the possible status of telomere length and its relation with spe...
متن کاملComparative proteomic analysis of spermatozoa isolated by swim-up or density gradient centrifugation
BACKGROUND Reports about the morphologic and functional characteristics of spermatozoa prepared by density gradient centrifugation (DC) or swim-up (SU) have produced discordant results. We have performed a proteomic comparison of cells prepared by DC and SU providing a molecular insight into the differences between these two methods of sperm cell isolation. METHODS Protein maps were obtained ...
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ژورنال
عنوان ژورنال: Andrology
سال: 2017
ISSN: 2047-2919
DOI: 10.1111/andr.12372